The hugely popular Bolt was given a standing ovation after the individual 100-meters final, when a bitterly disappointed crowd watched as Gatlin took the gold.
They say anything can happen in the 4x100m relay but truth is no one expected this: Bolt looked certain to add a medal of some colour to his already 11 World Championship gold medals won over the last decade, two silvers, and not forgetting the bronze medal won in the 100m final last weekend.
Jamaica recorded a season best time of 37.95 seconds in the 4 X 100 m relay Heats on Saturday and in the finals, Bolt and the rest of the Jamaican team comprising Tyquendo Tracey, Julian Forte and Micheal Campbell will aim to do better than the United States or Great Britain. Gatlin revealed afterwards he thought the cold was likely to have played a part in Bolt's injury while Jamaica's Yohan Blake could not contain his anger that organisers had allowed the women's high jump medal ceremony to delay the start of the race. Covering the first 60 metres in an unprecedented 6.31 seconds, Bolt did not ease up and in 41 strides streaked through the finish line in an awesome world record of 9.58sec, a mark that will likely be unchallenged for many years to come.
Brilliantly executed exchanges by Chijindu Ujah, Adam Gemili, Danny Talbot and Nethaneel Mitchell-Blake gave Britain gold in 37.47 seconds, breaking an 18-year-old national record and European record and securing the world title for the first time. Hopefully, he'll be remembered for the successes in his career, rather than the disappointing end of it.
Before Farah, there are three gold medals up for grabs tonight.
"We've got to contend with the best athletes in the world".
Running several alternates on their team, the United States finished in 2 minutes, 59.23 seconds, .12 seconds ahead of Trinidad and Tobago.
The women's quartet, of Zoey Clark, Laviai Nielsen, Perri Shakes-Drayton and Emily Diamond, crossed in a time of 3:24.74 - one second faster than the time that won Team GB bronze in the event last summer. The German beat Jakub Vadlejch of the Czech Republic by 16 centimeters. Rico Freimuth took silver with 8,564 points, while German teammate Kai Kazmirek was third with 8,488.
In an eight-year period from 2008 to 2016, Bolt won 20 Olympic and world championship gold medals in the 21 events he entered.
"To run it with Danny Talbot after such disappointment in London 2012, it is so special to come back", said Gemili, who was not selected for the individual races and missed an Olympic 200m bronze by three thousandths of a second previous year.